Protein posttranslational modifications are key mechanisms in regulating protein activity and stability. Dr. Pandey’s lab has successfully used these quantitative proteomic strategies to study global protein phosphorylation, ubiquitination, acetylation and methylation. With these technologies, Dr. Pandey’s group has made seminal discoveries in multiple signaling pathways including EGFR/HER2, PI3K-AKT, RAS-RAF-MAPK and TSLP signaling. Cancer signaling research implying genetic engineering tools by either overexpressing or silencing of genes with the latest technologies like clustered regularly interspaced short palindromic repeat guide RNA associated with a Cas nuclease (CRISPR/Cas9).